Tuesday, April 22, 2008

Generalized Eukaryotic Cell (3)

* The functions of the major organelles/ Structure & functions of the plasma membrane
Cytosol - fluid component of the cytoplasm and consists of an aqueous solution ( proteins,nutrients, other solutes)

^Plasma membrane - regulates the passage of materials into and out of the cell.
- phospholipid bilayer with transport proteins embedded throughout - allow certain ions and polar molecules to pass through
- Cell adhesion molecules(CAMs) - cell recognition and adhesion.
- Receptors are complex proteins or glycoproteins embedded with sites that bind to specific molecules in the cell's external environment.

^Nucleus - contains the DNA, with structural proteins(histones) to form chromosomes.
- Nucleolus - dense structure in the nucelus where ribosomal RNA(rRNA) synthesis occurs.

^Ribosomes - sites of protein production & synthesized by the nucleolus. 2 subunits(Prokaryotes - 30S & 50S; Eukaryotes: 40S & 60S).

^Endoplasmic Reticulum - RER : with ribosomes - involved in protein synthesis
- SER : w/o ribosome - lipid synthesis; detoxification of drugs & poison

^Golgi Apparatus - Modify contents from SER, repack, redistribute. - secretory vesicles. Vesiciles/ Vacuoles.

^Lysosomes - pH5, contains hydrolytic enzymes.

^Microbodies - peroxisomes - oxidative enzymes. H2O2. Glyoxysomes to convert fats into sugars until seedling is mature to produce own sugars.

^Mitochondria - Site of aerobic respiration; supply of energy. Semiautonomous; contain their own DNA & ribosomes, self- replicate through binary fission.

^ Chloroplasts - contain chlorophyll, site of photosynthesis. Semiautonomous.

^Centrioles - specialized type of microtubue involved in spindle organization during cell division.

^Cytoskeleton -
- Microtubules - polymerized tubulins to provide support. Centrioles direct the separation of chromosomes during cell division. Cilia & Flagella - involved in cell motility.

- Microfilaments - actin in muscle contraction. amoeboid movement.

- Intermediate filament - maintenance of cytoskeletal integrity.

*How the size of a typical eukaryotic cell compares to the size of a bacterium or virus

Virus = 20 -300nm
Bacteria=1 - 10um
Eukaryotic cell = 10 -100um

*Osmosis, Passive transport, Active transport, Endocytosis, Exocytosis

Osmosis - simple diffusion from region of lower solute concentration to a region of higher solute concentrations through a selectively permeable membrane.

^Passive transport - down gradient, No carrier, No energy required
^Facilitated transport - down gradient, carrier, No Energy required
^Active Transport - against gradient, Carrier, Energy required
^Endocytosis - process in which teh cell membrane invaginates, forming a vesicle.

*Structure & functions of the cytoskeleton

General function - cell mechanical support, maintain it's shape, function in cell motility.

^Microtubules - hollow polymerized tubulins that radiate throughout the cell & provide support. (Etc: centrioles- direct the separation of chromosomes during cell division) ( Cilia & flagella - involved in cell motility).

^Microfilament - actin- involved in cell movement as well as support. Myosin- actin interaction.

^Intermediate filaments - involved in maintenance of cytoskeletal integrity.

*Mitosis : where it falls in the cell cycle